WB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDa
MeCP2 Recombinant Rabbit mAb (S-1884-28)
MeCP2 Recombinant Rabbit mAb (S-1884-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MeCP2 |
| Synonyms | Methyl-CpG-binding protein 2; MeCp-2 protein |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P51608 |
| Clone Number | S-1884-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Rt |
| Positive Sample | SH-SY5Y, Neuro-2a, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Rt |
Background
Methyl-CpG-binding protein 2 (MeCP2) is a crucial nuclear protein predominantly found in mature neurons, initially identified as a transcriptional repressor that recognizes DNA methylation marks. It plays a significant role in neurodevelopment, with mutations in the MECP2 gene causing severe neurological disorders such as Rett syndrome (RTT) and MeCP2 duplication syndrome (MDS), which are characterized by progressive neurological dysfunction and developmental regression. MeCP2 exhibits a preference for binding methylated cytosines in both CpG and non-CpG contexts and can also bind unmethylated DNA. It is involved in chromatin organization and can interact with nucleosomes and other effector proteins, such as the NCoR1/2 co-repressor complex, to regulate gene expression. Recent research suggests that MeCP2's functions are more complex than previously thought, with its roles in neurodevelopment and adult brain function linked to its ability to act as a global chromatin regulator.
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Western Blot
WB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/500 dilution
Lane 1: Neuro-2a nuclear fraction lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDa
WB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDa
WB result of MeCP2 Recombinant Rabbit mAb
Primary antibody: MeCP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 80 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MeCP2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
