WB result of MBD3 Rabbit pAb
Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: NTERA-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
MBD3 Rabbit Polyclonal Antibody
MBD3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MBD3 |
| Synonyms | Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | O95983 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | 293T, NTERA-2, F9, C6, rat testis, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Ms, Rt |
Background
MBD3 is a 32-kDa core subunit of the nuclear Mi-2/NuRD chromatin-remodeling complex that is essential for embryonic development in mice; it binds 5-methyl-cytosine DNA indirectly through a degenerate methyl-CpG-binding domain, couples ATP-dependent nucleosome sliding with histone deacetylase activity, and thereby orchestrates transcriptional repression, pluripotency exit, lineage specification, and heterochromatin formation across vertebrate genomes.
Picture
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Western Blot
WB result of MBD3 Rabbit pAb
Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
WB result of MBD3 Rabbit pAb
Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
WB result of MBD3 Rabbit pAb
Primary antibody: MBD3 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 33 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-MBD3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
