IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MAP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
MAP2 Recombinant Rabbit mAb (SDT-2060-28)
MAP2 Recombinant Rabbit mAb (SDT-2060-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | MAP2 |
| Synonyms | Microtubule-associated protein 2; MAP-2 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P11137 |
| Clone Number | S-2060-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Hu, Ms, Rt |
Background
Microtubule-associated protein 2 (MAP2) is a neuronal protein that plays a crucial role in the development and maintenance of the nervous system. It is primarily found in the dendrites and cell bodies of neurons, where it helps to stabilize and organize microtubules, which are essential components of the cytoskeleton. This stabilization is vital for maintaining the structural integrity of neurons and facilitating the transport of various molecules and organelles within the cell. MAP2 is also involved in synaptic plasticity, the ability of synapses to strengthen or weaken over time, which is a key process in learning and memory. Additionally, it has been implicated in various neurodegenerative diseases, as alterations in MAP2 levels or function can lead to neuronal dysfunction and cell death. Its expression is often used as a marker for neuronal differentiation and maturation in research settings.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human neuroblastoma. Anti-MAP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MAP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MAP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-MAP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-MAP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-MAP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
