WB result of M-Cadherin/Cadherin 15 Recombinant Rabbit mAb
Primary antibody: M-Cadherin/Cadherin 15 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 85, 110, 135 kDa
This blot was developed with high sensitivity substrate
M-Cadherin/Cadherin 15 Recombinant Rabbit mAb (S-2261-74)
M-Cadherin/Cadherin 15 Recombinant Rabbit mAb (S-2261-74)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | M-Cadherin/Cadherin 15 |
| Synonyms | Cadherin-15; Cadherin-14; Muscle cadherin (M-cadherin); Cdh14; Cdh15 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P33146 |
| Clone Number | S-2261-74 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Ms |
| Positive Sample | C2C12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| ICC | 1:500 | Ms |
Background
M-Cadherin (Cadherin-15), a classical calcium-dependent cell-adhesion glycoprotein, is primarily expressed in developing skeletal muscle, satellite cells and cerebellum, where it mediates homophilic binding to organize myoblast fusion, muscle fiber formation and neuronal layer integrity by assembling adherens junctions through catenin-dependent linkage to the actin cytoskeleton ; despite its name, germline deletion in mice is largely compensated by N-cadherin, yet human mutations causing loss of adhesive function are linked to autosomal-dominant mental retardation-3, underscoring a non-redundant role in synaptic and cognitive development .
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in C2C12 cells (top panel) and negative staining in NIH/3T3 cells (below panel). Anti- M-cadherin/cadherin-15 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
