WB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
LMPTP Rabbit Polyclonal Antibody
LMPTP Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | LMPTP |
| Synonyms | Low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase; Red cell acid phosphatase 1; ACP1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P24666 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, K562, HeLa, HepG2, NIH/3T3, mouse brain, C6, rat liver, COS-7 |
| Predicted Reactivity | Ck, Pg, Or, Bv, Dr |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Background
Low molecular weight protein tyrosine phosphatase (LMPTP), an 18 kDa cytosolic class II PTP encoded by the ACP1 gene, exists as two splice isoforms (LMPTP-A and LMPTP-B), is ubiquitously expressed with especially high levels in adipocytes, and functions as a negative regulator of insulin signalling by dephosphorylating the insulin receptor and its substrates; human genetic studies show that low-activity ACP1 alleles are associated with reduced glycaemia, while experimental knockdown or genetic deletion of LMPTP in obese mice improves glucose tolerance and insulin sensitivity—mainly via hepatic effects—demonstrating that LMPTP drives obesity-linked insulin resistance and type 2 diabetes, thus representing an attractive drug target for metabolic disorders.
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Western Blot
WB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17, 18 kDa
WB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 17, 18 kDa
WB result of LMPTP Rabbit pAb
Primary antibody: LMPTP Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-LMPTP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HEK293 cells. Anti-LMPTP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
