Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse MHC Class I (H-2Kb) antibody at 1/500 (1 μg) dilution (Right panel) compared with a Rat IgG2b, κ Isotype Control (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45R/B220 - Brilliant Violet 421™ antibody separately.
Invivo Anti-Mouse MHC Class I (H-2Kb) Recombinant mAb
Invivo Anti-Mouse MHC Class I (H-2Kb) Recombinant mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | MHC Class I (H-2Kb) |
| Synonyms | H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1 |
| Accession | P01901 |
| Antibody Type | Mouse mAb |
| Isotype | Rat IgG2b,k |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 5 mg/ml |
| Purity | >95% by SEC-HPLC |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 2 to 8 °C for 2 weeks under sterile conditions; |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:500 | Ms |
Background
MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.
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