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Invivo Anti-Mouse CD40 Recombinant mAb (S-R456)

Invivo Anti-Mouse CD40 Recombinant mAb (S-R456)

Catalog Number: S0B7000 Application: FCM,Functional/Block/Neutralize Reactivity: Mouse Conjugation: Unconjugated Brand: Starter
Price:
Regular price $150.00 USD
Regular price Sale price $150.00 USD
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Product Details

Product Specification


Host Rat
Antigen CD40
Synonyms Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; Tnfrsf5
Location Secreted, Cell membrane
Accession P27512
Clone Number S-R456
Antibody Type Rat mAb
Isotype Rat IgG2a,k
Application FCM, in vivo CD40 activation, in vitro B cell stimulation/activation
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein G
Concentration 5 mg/ml
Endotoxin <2EU/mg
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS pH7.4

Stability & Storage

2 to 8 °C for 2 weeks under sterile conditions;

-20 °C for 3 months under sterile conditions; 

-80 °C for 24 months under sterile conditions.

Please avoid repeated freeze-thaw cycles.

Dilution


application dilution species
FCM 1:250 Ms

Background

This antibody recognizes the same epitope as clone FGK45. CD40, also known as TNFRSF5, is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily, primarily expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. Its ligand, CD40L (CD154), is mainly found on activated T cells and is also expressed on platelets, endothelial cells, and smooth muscle cells. The interaction between CD40 and CD40L plays a crucial role in immune regulation, mediating a wide range of immune responses, including B cell proliferation, differentiation, and immunoglobulin class switching, as well as enhancing T cell activation and dendritic cell maturation. This interaction initiates intracellular signaling pathways involving TRAF proteins and leads to the activation of transcription factors such as NF-κB, which in turn regulate cytokine production and immune cell function. CD40 signaling is also implicated in metabolic processes, influencing glucose metabolism and lipid homeostasis, particularly in macrophages and endothelial cells. Dysregulation of the CD40-CD40L pathway is associated with various diseases, including immunodeficiency syndromes and autoimmune disorders.

Picture

FC

Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD40 antibody at 1/250 (2 μg) dilution (Right) compared with a Rat IgG2a, κ isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.

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