Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD40 antibody at 1/250 (2 μg) dilution (Right) compared with a Rat IgG2a, κ isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Alexa Fluor® 647 separately. Gated on total viable cells.
Product Details
Product Details
Product Specification
Host | Rat |
Antigen | CD40 |
Synonyms | Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; Tnfrsf5 |
Location | Secreted, Cell membrane |
Accession | P27512 |
Clone Number | S-R456 |
Antibody Type | Rat mAb |
Isotype | Rat IgG2a,k |
Application | FCM, in vivo CD40 activation, in vitro B cell stimulation/activation |
Reactivity | Ms |
Positive Sample | C57BL/6 mouse splenocytes |
Purification | Protein G |
Concentration | 5 mg/ml |
Endotoxin | <2EU/mg |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS pH7.4 |
Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
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Dilution
application | dilution | species |
FCM | 1:250 | Ms |
Background
This antibody recognizes the same epitope as clone FGK45. CD40, also known as TNFRSF5, is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily, primarily expressed on antigen-presenting cells such as dendritic cells, macrophages, and B cells. Its ligand, CD40L (CD154), is mainly found on activated T cells and is also expressed on platelets, endothelial cells, and smooth muscle cells. The interaction between CD40 and CD40L plays a crucial role in immune regulation, mediating a wide range of immune responses, including B cell proliferation, differentiation, and immunoglobulin class switching, as well as enhancing T cell activation and dendritic cell maturation. This interaction initiates intracellular signaling pathways involving TRAF proteins and leads to the activation of transcription factors such as NF-κB, which in turn regulate cytokine production and immune cell function. CD40 signaling is also implicated in metabolic processes, influencing glucose metabolism and lipid homeostasis, particularly in macrophages and endothelial cells. Dysregulation of the CD40-CD40L pathway is associated with various diseases, including immunodeficiency syndromes and autoimmune disorders.
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