WB result of IL-18 Recombinant Rabbit mAb
Primary antibody: IL-18 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: PC-3 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: DU 145 whole cell lysate 20 µg
Negative control: Raji whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 20 kDa
IL-18 Recombinant Rabbit mAb (S-2142-2)
IL-18 Recombinant Rabbit mAb (S-2142-2)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-18 |
| Synonyms | Interleukin-18; Iboctadekin; Interferon gamma-inducing factor (IFN-gamma-inducing factor); Interleukin-1 gamma (IL-1 gamma); IGIF; IL1F4; IL18 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Secreted |
| Accession | Q14116 |
| Clone Number | S-2142-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | PC-3, A431, HeLa, DU 145 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
Interleukin-18 (IL-18) is a 18 kDa pro-inflammatory cytokine of the IL-1 superfamily, constitutively expressed by macrophages, dendritic cells, and epithelial cells as an inactive 24 kDa pro-peptide that is cleaved by caspase-1 within the inflammasome to its mature form; once secreted, IL-18 binds the heterodimeric receptor IL-18Rα/IL-18Rβ and recruits the adaptor MyD88 to activate NF-κB and MAPK pathways, synergizing with IL-12 to drive IFN-γ production from Th1 cells and NK cells and promote cell-mediated immunity, but when unchecked it contributes to the pathogenesis of autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease, and type 1 diabetes, making it both a biomarker and therapeutic target.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- IL-18 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
