WB result of IL-1 beta Recombinant Rabbit mAb
Primary antibody: IL-1 beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 100 ng/ml LPS for 6 hours, then add 300 ng/ml Brefeldin A for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 37 kDa
IL-1 beta Recombinant Rabbit mAb (S-3173)
IL-1 beta Recombinant Rabbit mAb (S-3173)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | IL-1 beta |
| Synonyms | Interleukin-1 beta; Catabolin; IL1F2; IL1B |
| Location | Cytoplasm, Secreted, Lysosome |
| Accession | P01584 |
| Clone Number | S-3173 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Ms |
| ICC | 1:100 | Hu |
Background
Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is produced by various cell types, including macrophages and monocytes, in response to infections or tissue injury. IL-1β helps to initiate and amplify the inflammatory response by promoting the expression of other inflammatory mediators and recruiting immune cells to the site of infection or damage. It also has effects on a wide range of physiological processes, such as fever induction, modulation of the hypothalamic-pituitary-adrenal axis, and regulation of metabolism. Dysregulation of IL-1β has been implicated in numerous chronic inflammatory diseases, like rheumatoid arthritis and inflammatory bowel disease, making it an important target for therapeutic interventions.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of THP-1 cells treated with TPA (80 nM,16h), then add LPS (100 ng/ml,6h), then add BFA (300 ng/ml, 3h) (top panel) and untreated THP-1 cells (below panel). Anti- IL-1 beta antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
