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IdeZ Protease (Tag Free)

IdeZ Protease (Tag Free)

Catalog Number: UA070033 Brand: UA BIOSCIENCE
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$462 USD
$462 USD
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Product Details

Product Specification


Species Streptococcus equi subsp. Zooepidemicus
Synonyms IdeZ
Expression System E.coli
Molecular Weight

36kDa (Reducing)
Purity ＞95% by SDS-PAGE
Conjugation Unconjugated
Tag No Tag
Physical Appearance Liquid
Storage Buffer Tris-HC, pH7.5.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied; 

1 week, 2 to 8 °C under sterile conditions; 

Please avoid repeated freeze-thaw cycles.
Reference

[1] Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZProtease (Tag Free) in Glycan Profiling of Therapeutic Antibodies[J].Glycobiology, 2016(12):26.

[2]Cheng-Wei H, JieP, Yu-Qing O ,et al. Characteristics of IgG degradation enzymeIdeZ in Streptococcus equi ssp. zooepidimicus[J].Chinese Journal of PreventiveVeterinary Medicine, 2018..

Background

IdeZ Protease secreted by the Streptococcus equisubspecies zooepidemicus. This product is an engineered recombinant proteaseover expressed in Escherichia coli. It can specifically identify IgG, andperform enzyme digestion at specific sites in the hinge region of the antibody,and produce F(ab')2 fragments and Fc fragments, which can identify human and otheranimal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG,etc, It can be used for structural characterization analysis of antibodies andfusion protein drugs.

Components

Protocol

1. Add the desired amount of 5mg IgG indigestion buffer or other compatible buffer*.

2. Add IdeZ Protease (Tag Free)-1 to thereaction system:

• Add 1 unit of IdeZ Protease (Tag Free)-1 per1µg of IgG to be digested.

• For example, add 5µl (200 units) ofreconstituted IdeZ to digest 200µg of IgG.

3. Incubate sample at 37°C for 30–60minutes

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).