2μg (R: reducing condition, N: non-reducing condition).
IdeZ Protease (Tag Free)
IdeZ Protease (Tag Free)
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$462 USD
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Product Details
Product Details
Product Specification
| Species | Streptococcus equi subsp. Zooepidemicus |
| Synonyms | IdeZ |
| Expression System | E.coli |
| Molecular Weight | 36kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Liquid |
| Storage Buffer | Tris-HC, pH7.5. |
| Stability & Storage |
12 months from date of receipt, -20 to -70 °C as supplied; |
| Reference |
[1] Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease (Tag Free) in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26. [2] Cheng-Wei H, JieP, Yu-Qing O ,et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J].Chinese Journal of Preventive Veterinary Medicine, 2018.. |
Background
IdeZ Protease secreted by the Streptococcus equi
subspecies zooepidemicus. This product is an engineered recombinant protease
over expressed in Escherichia coli. It can specifically identify IgG, and
perform enzyme digestion at specific sites in the hinge region of the antibody,
and produce F(ab')2 fragments and Fc fragments, which can identify human and other
animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG,
etc, It can be used for structural characterization analysis of antibodies and
fusion protein drugs.
Components
Protocol
1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.
2. Add IdeZ Protease (Tag Free)-1 to the reaction system:
• Add 1 unit of IdeZ Protease (Tag Free)-1 per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.
3. Incubate sample at 37°C for 30–60 minutes
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C
Picture
Picture
SDS-PAGE
