| Species | Streptococcus pyogenes |
| Synonyms | IdeS、IdeS Protease、Immunoglubulin-degrading enzyme |
| Expression System | E.coli |
| Molecular Weight | 38 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS pH 6.6 |
| Stability & Storage |
·12 months from date of receipt, -20 to -70 °C as supplied. · It can be stored at 4-8℃ for one week after dissolution, under sterile conditions · Please avoid repeated freeze-thaw cycles. |
| Reference |
[2] Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615. |
IdeS Protease is a cysteine hydrolase secreted
by the human pathogen Streptococcus pyogenes. This product use E. coli
recombinant expression, high purity, has good enzyme digestion activity, can
specifically identify IgG, and perform enzyme digestion at specific sites in
the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2
fragments and Fc fragments, which can identify human and other animal IgG, such
as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be
used for structural characterization analysis of antibodies and fusion protein
drugs
1. Add the desired amount of 5mg IgG in digestion buffer or other compatible
buffer * .
2. Add IdeS Protease(Tag Free) to the reaction system:
• Add 1 unit of IdeS Protease(Tag Free) per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.
3. Incubate sample at 37°C for 30–60 minutes
The results of 5μg rabbit IgG digestion separated under different quantity of IdeS Protease(Tag Free), The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS Protease (Tag Free);
lane 3 rabbit IgG add 10units IdeS Protease (Tag Free);
lane 4 rabbit IgG add 16.7 units IdeS Protease (Tag Free);
lane 5 rabbit IgG add 50 units IdeS Protease (Tag Free);
2μg (R: reducing condition, N: non-reducing
condition).
