| Species | Streptococcus pyogenes |
| Synonyms | IdeS、IdeS Protease、Immunoglubulin-degrading enzyme |
| Expression System | E.coli |
| Molecular Weight | 38 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS pH 6.6 |
| Stability & Storage |
·12 months from date of receipt, -20 to -70 °C as supplied. · It can be stored at 4-8℃ for one week after dissolution, under sterile conditions · Please avoid repeated freeze-thaw cycles. |
| Reference |
[2] Pawel-Rammingen V, U. IdeS, a novelstreptococcal cysteine proteinase with unique specificity for immunoglobulinG[J]. Embo Journal, 2014, 21(7):1607-1615. |
IdeS Protease is a cysteine hydrolase secretedby the human pathogen Streptococcus pyogenes. This product use E. colirecombinant expression, high purity, has good enzyme digestion activity, canspecifically identify IgG, and perform enzyme digestion at specific sites inthe hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2fragments and Fc fragments, which can identify human and other animal IgG, suchas mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can beused for structural characterization analysis of antibodies and fusion proteindrugs
1. Add the desired amount of 5mg IgG in digestionbuffer or other compatible
buffer*.
2. Add IdeS Protease(Tag Free) tothe reaction system:
•Add 1 unit of IdeS Protease(Tag Free) per 1µg of IgG to be digested.
•For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.
3. Incubate sample at 37°C for30–60 minutes
The results of 5μg rabbit IgG digestion separated under different quantity of IdeS Protease(Tag Free), The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).
M, marker;
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeS Protease (Tag Free);
lane 3 rabbit IgG add 10units IdeS Protease (Tag Free);
lane 4 rabbit IgG add 16.7 units IdeS Protease (Tag Free);
lane 5 rabbit IgG add 50 units IdeS Protease (Tag Free);
2μg (R: reducing condition, N: non-reducing
condition).
