Standard curve
Example of Human TGF-β1 standard curve in Assay Diluent #11D.
Human/Monkey/Mouse/Rat/Rabbit/Porcine/Goat/Bovine/Horse/Donkey /Feline/Canine TGF-β1 OneStep ELISA Kit
Human/Monkey/Mouse/Rat/Rabbit/Porcine/Goat/Bovine/Horse/Donkey /Feline/Canine TGF-β1 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | TGF-β1 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 6.1%； Inter-assay: 9.7% |
| Sample type | Cell culture supernatant, Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 10.58 pg/ml |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 82% Serum: 107% Plasma: 101% |
| Assay time | 60 minutes |
| Species reactivity | Hu; Mk; Ms; Rt; Rb; Donkey; Goat; Horse; Bovine; Porcine; Feline; Canine |
Background
Human Transforming Growth Factor-beta 1 (TGF-β1) is a highly conserved, disulfide-linked homodimeric cytokine (25 kDa) that plays crucial roles in cell proliferation, differentiation, and immune regulation. It is synthesized as a latent precursor consisting of a signal peptide, latency-associated peptide (LAP), and the mature TGF-β1 domain. The mature bioactive form is released from LAP through proteolytic cleavage or acid treatment (pH ≤ 3.0), which disrupts non-covalent interactions while preserving the dimer's structural integrity via critical disulfide bonds. Human TGF-β1 shares the following identity with other species: Monkey (Macaca spp.): ~99%; Mouse (Mus musculus): ~98%; Rat (Rattus norvegicus): ~98%; Rabbit (Oryctolagus cuniculus): ~97%; Donkey (Equus asinus): ~96%; Goat (Capra hircus): ~95%; Horse (Equus caballus): ~96%. The high homology underscores TGF-β1’s critical role in fundamental biological processes across mammals, enabling translational research using animal models. However, species-specific post-translational modifications or receptor interactions may still influence functional outcomes.
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ELISA
Linearity
The concentrations of Human TGF-β1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells culture for 3days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1185.88 pg/mL in unstimulated human PBMC supernatant.
Linearity
The concentrations of Human TGF-β1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human serum (2.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 49.67 ng/mL in human serum.
Linearity
The concentrations of Human TGF-β1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Human plasma (12.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.72 ng/mL in human plasma.
Linearity
The concentrations of TGF-β1 were measured and interpolated from the target standard curves and corrected for sample dilution.
Monkey serum (1.25%), Mouse serum(0.625%), Rat serum(1.25%), Rabbit serum(5%), Donkey serum(2.5%), Goat serum(1.25%), Horse serum(5%), Porcine serum(2.5%), Feline serum(5%) and Canine serum(5%). The interplated dilution factor corrected values are plotted. The mean target concentration was determined to be 65.10 ng/mL in Monkey serum. The mean target concentration was determined to be 149.10 ng/mL in Mouse serum. The mean target concentration was determined to be 106.28 ng/mL in Rat serum. The mean target concentration was determined to be 6.15 ng/mL in Rabbit serum. The mean target concentration was determined to be 8.12 ng/mL in Donkey serum. The mean target concentration was determined to be 16.25 ng/mL in Goat serum. The mean target concentration was determined to be 11.24 ng/mL in horse serum. The mean target concentration was determined to be 26.30 ng/mL in porcine serum. The mean target concentration was determined to be 8.88 ng/mL in feline serum. The mean target concentration was determined to be 12.49 ng/mL in canine serum.
Specificity
Test the different standards. The human TGF-β1 standard curve ranged from 15.63 pg/mL to 1000 pg/mL. The human
TGF-β2 (Cat: UA040387) standard curve ranged from 156.3 pg/mL to 10000 pg/mL.
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Protocol Diagram
