Standard curve
Example of Human TNF-β standard curve in Assay Diluent #12.
Human TNF-β OneStep ELISA kits
Human TNF-β OneStep ELISA kits
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Product Details
Product Details
Product Specification
| Antigen | TNF-β |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 5.7%； Inter-assay: 7.5% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.110 ng/mL |
| Range | 0.156 pg/mL – 10 ng/mL |
| Recovery | Cell culture supernatant: 86% Serum: 111% Plasma: 115% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Human Tumor Necrosis Factor-beta (TNF-β), also known as lymphotoxin-alpha (LT-α), is a pro-inflammatory cytokine primarily secreted by activated lymphocytes, including T cells, B cells, and NK cells. It signals through the TNF receptor 1 (TNFR1) and TNFR2, activating downstream pathways such as NF-κB, MAPK, and apoptosis cascades, which regulate immune responses and inflammation. Unlike TNF-α, which is produced by macrophages and other innate immune cells, TNF-β plays a specialized role in lymphoid organ development, host defense, and autoimmune pathogenesis. It contributes to the formation of secondary lymphoid structures and promotes cytotoxic T cell and B cell functions. Dysregulated TNF-β expression is associated with chronic inflammatory diseases, including rheumatoid arthritis and multiple sclerosis, making it a potential therapeutic target. Its dual role in immune regulation and pathology underscores its significance in both physiological and disease contexts.
Picture
Picture
ELISA
Linearity
The concentrations of Human TNF-β were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be1.69 ng/mL in stimulated human PBMC supernatant.
To assess the linearity of the assay, the samples was spiked with high concentrations of Human TNF-β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human TNF-β in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
