Standard curve
Example of Human MCP-1 standard curve in Assay Diluent #11.
Human MCP-1 OneStep ELISA Kit
Human MCP-1 OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | MCP-1 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.8%； Inter-assay: 3.7% |
| Sample type | Cell culture supernatant, Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.716 pg/ml |
| Range | 1.56 pg/mL – 100 pg/mL |
| Recovery | Cell culture supernatant: 101% Serum: 102% Plasma: 87% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
MCP-1, also known as CCL2, is a chemokine belonging to the C-C chemokine family. It plays a crucial role in various physiological processes such as inflammation, immune regulation, and cell migration. MCP-1/CCL2 primarily functions by binding to the C-C chemokine receptor CCR2, thereby guiding monocytes and other inflammatory cells to migrate to sites of inflammation, contributing to the inflammatory response and immune regulation. Overexpression of MCP-1/CCL2 is closely associated with the pathogenesis and progression of various diseases, including inflammatory diseases, autoimmune disorders, cancer, and metabolic disorders. As a result, MCP-1/CCL2 has emerged as a potential therapeutic target, and researchers are exploring therapeutic strategies targeting MCP-1/CCL2 in the hope of impacting the treatment of related diseases.
Picture
Picture
ELISA
Linearity
The concentrations of MCP-1were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human Serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 218 pg/mL in Human Serum.
#2 sample is undiluted samples are as follows: Human Plasma was 1.3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 103 pg/mL in Human Plasma.
Linearity
The concentrations of MCP-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 1days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 8690 pg/mL in stimulated human PBMC supernatant.
Linearity
The sample is undiluted samples are as follows: human PBMC cells culture for 1days (4%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2930 pg/mL in unstimulated human PBMC supernatant.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human MCP-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, the samples were spiked with high concentrations of Human MCP-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram
