2μg（R: reducing conditions）
Human Intrinsic Factor/CBLIF Protein, His tag
Human Intrinsic Factor/CBLIF Protein, His tag
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Cobalamin binding intrinsic factor, Gastric intrinsic factor, Intrinsic factor (IF; INF), GIF, IFMH |
| Accession | P27352 |
| Amino Acid Sequence | Protein sequence (P27352, Ser19-Try417, with C-His tag) STQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 45.1 kDa Observed MW: 54 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | with C-His tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Cobalamin binding intrinsic factor is a glycoprotein produced by the parietal cells of the stomach. Its main function is to bind to vitamin B₁₂ (cobalamin) in the digestive tract. This binding protects cobalamin from degradation and facilitates its absorption in the ileum of the small intestine. The complex of intrinsic factor and cobalamin is recognized by specific receptors on the ileal epithelial cells, allowing for the efficient uptake of vitamin B₁₂ into the bloodstream. Deficiency of intrinsic factor can lead to pernicious anemia, as it impairs the body's ability to absorb vitamin B₁₂, which is essential for normal hematopoiesis and neurological function.
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