Standard curve
Example of Human IL-4 standard curve in Assay Diluent #1.
Human IL-4 OneStep ELISA Kit
Human IL-4 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-4 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 4.1%； Inter-assay: 4.4% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.435 pg/ml |
| Range | 1.25 pg/mL – 80 pg/mL |
| Recovery | Cell culture supernatant: 87% Serum: 111% Plasma: 116% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| NIBSC convert | The dose response curve of the International Standard (88/656) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (88/656) units, use the following equation: NIBSC (88/656) approximate value (IU/mL) = 0.01 x OneStep Human IL-4 value (pg/mL). |
Background
Interleukin 4(IL4) was first identified as a helper T cell product with the capacity to co-stimulate B cell growth in vitro. IL-4 also can rescue B-cells from apoptosis, enhancing their survival, and is responsible for immunoglobulin isotype switching to IgG1 and IgE. The effect of IL-4 signaling is mediated through the IL-4 receptor alpha chain (IL-4Rα). Upon binding to its ligand, IL-4Rα dimerizes either with the common gamma chain (γc) to produce the type-1 signaling complex located mainly on hematopoietic cells, or with the IL-13 receptor alpha 1 (IL-13Rα1) to produce the type-2 complex, which is expressed also on non-hematopoietic cells. The type-1 signaling complex is critical for Th2-skewing of T cells and the development of alternatively activated macrophages (AAMΦs), while the type-2 complex plays a role in non-hematopoietic responses to IL-4 and IL-13.
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ELISA
Linearity
The concentrations of Human IL-4 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated or unstimulated with 10ug/ml PHA for 2days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.97 pg/mL in stimulated human PBMC supernatant, and unstimulated human PBMC supernatants did not contain.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-4 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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