WB result of hnRNP U Recombinant Rabbit mAb
Primary antibody: hnRNP U Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 120 kDa
hnRNP U Recombinant Rabbit mAb (S-2375-23)
hnRNP U Recombinant Rabbit mAb (S-2375-23)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | hnRNP U |
| Synonyms | Heterogeneous nuclear ribonucleoprotein U; GRIP120; Nuclear p120 ribonucleoprotein; Scaffold-attachment factor A (SAF-A); p120; pp120; C1orf199; HNRPU; SAFA; U21.1; HNRNPU |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q00839 |
| Clone Number | S-2375-23 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, 293T, K562, NIH/3T3, mouse liver, mouse brain, C6, rat brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:50000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000-1:5000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
| ICFCM | 1:50 | Hu, Ms |
Background
Heterogeneous nuclear ribonucleoprotein U (HNRNPU, also called scaffold attachment factor A/SAF-A) is a ~120 kDa, ubiquitously expressed nuclear protein that functions as a multifunctional hub integrating RNA metabolism, chromatin organization and transcriptional control: it binds both RNA and AT-rich scaffold/matrix attachment region DNA through N-terminal SAP and C-terminal RGG RNA-binding domains to organize 3-D chromatin architecture, regulate alternative pre-mRNA splicing by controlling U2 snRNP maturation and Cajal body morphology, stabilize or destabilize specific mRNAs via 3′-UTR interactions, and modulate RNA polymerase II elongation together with cofactors like p300/PCAF; its actions are essential for heart development (cardiac-specific deletion causes lethal dilated cardiomyopathy with widespread splicing defects), early neuronal differentiation and brain function (mutations produce intellectual disability, autism spectrum disorder and epilepsy), and it also participates in X-chromosome inactivation, telomere maintenance and protein homeostasis by acting as a pseudosubstrate for SCF ubiquitin ligases.
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Western Blot
WB result of hnRNP U Recombinant Rabbit mAb
Primary antibody: hnRNP U Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 120 kDa
WB result of hnRNP U Recombinant Rabbit mAb
Primary antibody: hnRNP U Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 120 kDa
WB result of hnRNP U Recombinant Rabbit mAb
Primary antibody: hnRNP U Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 120 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling hnRNP U antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling hnRNP U antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear carcinoma. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-hnRNP U antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- hnRNP U antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HeLa cells. Anti- hnRNP U antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
