HMGB1 Protein,Human
HMGB1 Protein,Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | High Mobility Group Protein B1; High Mobility Group Protein 1; HMG-1; HMGB1; HMG1 |
| Accession | P09429 |
| Amino Acid Sequence |
Met1-Phe89
|
| Expression System | E.coli |
| Molecular Weight | 10.4 KDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50mM HEPES, 500mM NaCl, 0.5mMDTT, pH 7.9 . |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
|
Background
High mobility group protein B1 is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3.It Contains 2 HMG box DNA-binding domains entitled box A and box B and It is a highly negative-charged C terminus. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses.
