WB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
GSDMD Recombinant Rabbit mAb (S-3267)
GSDMD Recombinant Rabbit mAb (S-3267)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GSDMD |
| Synonyms | Gasdermin-D; Gasdermin domain-containing protein 1; DFNA5L; GSDMDC1 |
| Location | Cytoplasm, Secreted, Mitochondrion, Cell membrane |
| Accession | P57764 |
| Clone Number | S-3267 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
Background
Gasdermin D (GSDMD) is a crucial protein within the gasdermin family, playing a key role in executing pyroptosis, a form of lytic, inflammatory cell death. The full-length GSDMD protein is inactive and consists of a 31 kD N-terminal pore-forming domain and a 22 kD C-terminal suppression domain. Upon activation by caspases such as caspase-1, -4, -5, or -11, the interdomain linker is cleaved, releasing the N-terminal fragment (GSDMD-NT) from the inhibitory C-terminal domain. The freed GSDMD-NT translocates to the plasma membrane, oligomerizes, and forms pores with an inner diameter of approximately 10–14 nm, leading to cell lysis, cytokine release (such as IL-1β and IL-18), and disruption of ion and water balance. This pore formation is a critical step in mediating inflammation and immune responses. GSDMD is also implicated in various diseases, including sepsis, myocardial ischemia/reperfusion injury, and certain cancers. Research on selective GSDMD inhibitors is ongoing, aiming to block pyroptosis and reduce inflammation in pathological conditions.
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Western Blot
WB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
WB result of GSDMD Recombinant Rabbit mAb
Primary antibody: GSDMD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-GSDMD antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
