WB result of Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate)
Primary antibody: β-actin Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate) at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate)
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (HRP Conjugate)
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Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Rabbit IgG |
| Immunogen | Rabbit IgG |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Rb |
| Purification | Immunogen Affinity |
| Concentration | 1.5 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ELISA | 1:1000-1:3000 | Rb |
| WB | 1:5000-1:10000 | Rb |
Background
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment is a type of secondary antibody fragment that is widely utilized in various immunological assays due to its specific characteristics. Derived from immunoglobulins of goats that have been immunized against rabbit IgG, this fragment consists of the antigen-binding regions (Fab')2 of the antibody molecule, which includes both the heavy (H) and light (L) chains. The F(ab')2 fragment is obtained by enzymatic digestion of the whole IgG molecule, specifically using pepsin, which cleaves the molecule into pieces while retaining the antigen-binding sites.
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Picture
Western Blot
