Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling Human ILT4 (CD85d) antibody (S0B0867) at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) antibody at 1/2000 (0.1 μg) dilution was used as the secondary antibody.
Negative control: THP-1
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
Price:
Regular price
$310.00 USD
Regular price
Sale price
$310.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Goat |
| Antigen | Rabbit IgG |
| Antibody Type | Polyclonal antibody |
| Application | ICC, FC |
| Reactivity | Rb |
| Purification | Immunogen Affinity |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:2000 | |
| FCM | 1:2000 |
Background
Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment is a type of secondary antibody fragment that is widely utilized in various immunological assays due to its specific characteristics. Derived from immunoglobulins of goats that have been immunized against rabbit IgG, this fragment consists of the antigen-binding regions (Fab')2 of the antibody molecule, which includes both the heavy (H) and light (L) chains. The F(ab')2 fragment is obtained by enzymatic digestion of the whole IgG molecule, specifically using pepsin, which cleaves the molecule into pieces while retaining the antigen-binding sites.
Picture
Picture
FC
Immunocytochemistry
ICC shows positive staining in BxPC-3 cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Tissue factor antibody (S0B2323) was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as secondary antibody at 1/2000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
