| Host | Rabbit |
| Antigen | Glypican-3 |
| Synonyms | GTR2-2, MXR7，GPC3，OCI5 |
| Immunogen | N/A |
| Location | Cytoplasm, Cell membrane |
| Accession | P51654 |
| Clone Number | SDT-R032 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, FCM, IP, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| FCM | 1:500 | null |
| IP | 1:25 | null |
| IHC-P | 1:500 | null |
| WB | 1:1000 | null |
| ICC | 1:500 | null |
| IF | 1:200 | null |
Glypican-3 (GPC3) is a cell-surface glyco phosphatidylinositol (GPI)-anchored protein that belongs to the heparan sulfate (HS) proteoglycan family, which plays important roles in cell growth, differentiation and migration. Many studies have shown that GPC3 is highly expressed in HCC, while its expression is absent in most nonmalignant adult tissues. GPC3 is currently used as an informative immunohistochemical biomarker for HCC, and it is believed to be an attractive target for HCC therapy.
WB result of Glypican-3 Rabbit mAb
Primary antibody: Glypican-3 Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 69 kDa
Exposure time: 18s
Flow cytometric analysis of HepG2 cells labelling Glypican-3 antibody at 1/500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Glypican-3 Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Glypican-3 in 0.4mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using Glypican-3 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : HepG2 whole cell lysate 10µg(input)
Lane 2 : Glypican-3 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 65 kDa
Observed MW: 69 kDa
Exposure time: 20s
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glypican-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glypican-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glypican-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Glypican-3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ICC shows positive staining in HepG2 cells. Anti-Glypican-3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).
IF shows positive staining in paraffin-embedded human hepatocellualr carcinoma (case 1). Anti-Glypican-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human hepatocellualr carcinoma (case 2). Anti-Glypican-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded human liver. Anti-Glypican-3 antibody was used at 1/200 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
