WB result of GAD65 Recombinant Rabbit mAb
Primary antibody: GAD65 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65 kDa
GAD65 Recombinant Rabbit mAb (S-2318-47)
GAD65 Recombinant Rabbit mAb (S-2318-47)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GAD65 |
| Synonyms | Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase (GAD-65); Glutamate decarboxylase 65 kDa isoform; GAD2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cytoplasm |
| Accession | Q05329 |
| Clone Number | S-2318-47 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse brain, mouse cerebellum, rat brain, rat cerebellum |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Ms, Rt |
| IHC-P | 1:250 | Ms, Rt |
Background
Glutamic acid decarboxylase 65 (GAD65) is an enzyme that synthesizes the inhibitory neurotransmitter γ-aminobutyric acid (GABA) from glutamate, playing a crucial role in the central nervous system. It is one of the two isoforms of glutamic acid decarboxylase, the other being GAD67. GAD65 is particularly notable for its dynamic structure and its role as a major autoantigen in type 1 diabetes (T1D), with autoantibodies against it often present before the clinical onset of the disease. Structurally, GAD65 forms obligate functional dimers and consists of three domains: the N-terminal domain (NTD), the pyridoxal-5'-phosphate (PLP)-binding domain, and the C-terminal domain (CTD). Its catalytic loop (CL) is dynamic, which contrasts with the stable conformation of GAD67's CL.
Picture
Picture
Western Blot
WB result of GAD65 Recombinant Rabbit mAb
Primary antibody: GAD65 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-GAD65 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
