G-CSF Protein, Human
G-CSF Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | G-CSF, CSF3, C17orf33, CSF3OS, GCSF, colony stimulating factor 3, CSF-3, Granulocyte Colony-Stimulating Factor |
| Accession | P09919-2 |
| Amino Acid Sequence | Thr31-Pro204 |
| Expression System | E.coli |
| Molecular Weight | 19kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 150mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-0.4 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1、Takano H. et al. (2007) G-CSF therapy for acute myocardial infarction. Trends Pharmacol Sci. 28(10): 512-517. |
Background
Granulocyte-colony stimulating factor (G-CSF) is a hematopoietic growth factor that stimulates the proliferation and differentiation of neutrophil precursor cells and enhances the functional properties of mature neutrophils. The mature human G-CSF (hG-CSF) is a glycoprotein with a molecular weight of approximately 19 kDa (174 amino acids) produced mainly by activated monocytes and macrophages. In the present scenario, hG-CSF has become one of the most widely used hematopoietic growth factor because of its proven efficacy against different forms of neutropenia caused mainly due to chemotherapy, radiotherapy and organ transplants. Mostly, it is recommended for the recovery from neutropenia after chemotherapy. The ability of G-CSF to mobilize stem cells from the bone marrow has found clinical applications in allogeneic and autologous transplantations. Also, the discovery of G-CSF as a neuroprotectant in neurological diseases like Alzheimer’s and Parkinson disease has garnered greater interests of researchers in the recent years.
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Bioactivity
SDS-PAGE
ELISA
Immobilized G-CSF, Human (Cat. No.
UA040043) at 2.0μg/mL (100μL/well) can bind G-CSFR/CD114 Fc Chimera, Human
(Cat. No. UA010537) with EC50 of 0.017-0.019μg/ml.
Immobilized G-CSF, Human (Cat. No. UA040043) at 2.0μg/mL (100μL/well) can bind G-CSFR/CD114 His Tag, Mouse (Cat. No. UA010219) with EC50 of 0.31-0.47μg/ml.
