Fc γ RIV/CD16-2 His Tag Protein, Mouse

Catalog Number: UA020016 Reactivity: Mouse Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$470.00 SGD

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Product Details

Product Specification

Species	Mouse
Synonyms	Low affinity immunoglobulin gamma Fc region receptor IV；CD16-2；Fc γ RIV
Accession	A0A0B4J1G0-1
Amino Acid Sequence	Gly21-Gln203, with C-terminal His
Expression System	HEK293
Molecular Weight	28-35kDa(Reducing)
Purity	＞95%, by SDS-PAGE under reducing conditions
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.

Background

Fc γ RIV (Low affinity immunoglobulin gamma Fc region receptor IV), also known as CD16-2 (Fc γ RIV), belongs to the Fc γ receptor family. Fc γ receptors can be divided into three types: Fc γ RI (CD64), Fc γ RII (CD32) and Fc γ RIII (CD16). Fc γ RI (CD64) has a high affinity for IgG monomers, while Fc γ RII (CD32) and Fc γ RIII (CD16) have relatively low affinity for IgG. Human CD16 is encoded by two genes, Fc γ RIIIA and Fc γ RIIIB. The gene sequence of CD16-2 is more similar to human Fc γ RIIIA protein. Fc γ RIV binding with IgG2a can promote osteocyte differentiation, while binding with IgE can promote macrophage-mediated phagocytosis, antigen presentation and pro-inflammatory cytokines production.

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Bioactivity

Anti-His antibody Immobilized on CM5 Chip captured CD16-2 His Tag, Mouse (Cat. No. UA020016), can bind IgG1-Fc (UA050001) with an affinity constant of 0.177 μM as determined in SPR assay (Biacore T200).

Anti-His antibody Immobilized on CM5 Chip captured CD16-2 His Tag, Mouse (Cat. No. UA020016), can bind Pertuzumab with an affinity constant of 0.211 μM as determined in SPR assay (Biacore T200).