WB result of ERCC1 Recombinant Rabbit mAb
Primary antibody: ERCC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 37 kDa
This blot was developed with high sensitivity substrate
ERCC1 Recombinant Rabbit mAb (SDT-2458-29)
ERCC1 Recombinant Rabbit mAb (SDT-2458-29)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ERCC1 |
| Synonyms | DNA excision repair protein ERCC-1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P07992 |
| Clone Number | SDT-2458-29 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, HepG2, SK-BR-3, MCF7, T-47D |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:2000 | Hu |
Background
ERCC1 (Excision Repair Cross-Complementation Group 1) is a 297-amino-acid protein that, together with its obligate partner XPF (ERCC4), forms a structure-specific heterodimeric endonuclease essential for nucleotide excision repair (NER) of helix-distorting DNA lesions caused by UV light or bulky adducts and for interstrand crosslink repair; the ERCC1–XPF complex incises the damaged DNA strand on the 5′ side of the lesion, enabling excision of a ~24–32-nt oligonucleotide, and its expression level or polymorphisms (e.g., C118T, C8092A) are extensively studied as predictive biomarkers for resistance to platinum-based chemotherapy in lung, ovarian, and other solid tumors because reduced ERCC1-mediated DNA repair enhances platinum-induced cytotoxicity.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ERCC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SK-BR-3 cells. Anti-ERCC1 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
