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Discosoma sp RFP, His tag

Discosoma sp RFP, His tag

Catalog Number: S0A0065 Brand: Starter
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$70 USD
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Product Details

Product Specification


Species Discosoma sp
Accession Q9U6Y8
Amino Acid Sequence

Protein sequence (Q9U6Y8, Met1-Leu225, with C-10*His)MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFLGGGGSHHHHHHHHHH
Expression System E. coli
Molecular Weight Predicted MW: 27.6 kDaObserved MW: 9, 19, 27.6 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Tag with C-His Tag
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red. RFP is approximately 25.9 kDa. The excitation maximum is 558 nm, and the emission maximum is 583 nm. Genetic engineering has improved the utility of RFP by increasing the speed of fluorescent development and creating monomeric variants. Improved variants of RFP include mFruits (mCherry, mOrange, mRaspberry), mKO, TagRFP, mKate, mRuby, FusionRed, mScarlet and DsRed-Express.

Picture

SDS-PAGE

2μg（R: reducing conditions）