WB result of DHFR Recombinant Rabbit mAb
Primary antibody: DHFR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: U937 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
DHFR Recombinant Rabbit mAb (S-2542-9)
DHFR Recombinant Rabbit mAb (S-2542-9)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DHFR |
| Synonyms | Dihydrofolate reductase |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Mitochondrion |
| Accession | P00374 |
| Clone Number | S-2542-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Mk |
| Positive Sample | K562, HEK-293, HeLa, Jurkat, U937, Raji, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Mk |
| IHC-P | 1:250 | Hu |
Background
Dihydrofolate reductase (DHFR) is a small, ~21 kDa oxidoreductase that catalyzes the NADPH-dependent reduction of 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate, thereby regenerating the essential one-carbon carrier required for de novo purine and thymidylate synthesis, making DHFR indispensable for DNA replication and cell proliferation, and its active site, containing a deeply bound nicotinamide and a flexible Met20 loop that alternates between closed and occluded conformations to control cofactor and substrate access, has been exploited for over seven decades by antifolate drugs such as methotrexate, pemetrexed, and trimethoprim that competitively block the folate-binding pocket, with differential binding kinetics and species-specific residue changes underpinning selective toxicity toward rapidly dividing cancer cells, bacteria, and parasites, while human DHFR variants (e.g., A19V, S59G, R28W) can confer resistance to antifolates, and beyond its canonical role DHFR is also implicated in oxidative stress responses, mitochondrial folate metabolism, and as a transcriptional regulator, with post-translational modifications like phosphorylation and nitrosylation modulating its stability and subcellular localization, and its expression levels serving as prognostic markers in leukemia, breast, and lung cancers, thus continuing to position DHFR as a central hub in both basic folate biochemistry and clinically relevant chemotherapy and antimicrobial strategies.
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Western Blot
WB result of DHFR Recombinant Rabbit mAb
Primary antibody: DHFR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-DHFR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
