WB result of CYP3A4 Recombinant Rabbit mAb
Primary antibody: CYP3A4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50 kDa
CYP3A4 Recombinant Rabbit mAb (S-2281-146)
CYP3A4 Recombinant Rabbit mAb (S-2281-146)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CYP3A4 |
| Synonyms | Cytochrome P450 3A4; 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; CYPIIIA3; CYP3A3 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | P08684 |
| Clone Number | S-2281-146 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms |
| Positive Sample | mouse liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:2000 | Hu, Ms |
| IF | 1:500 | Hu |
Background
CYP3A4 is a 57-kDa cytochrome P450 mono-oxygenase anchored in the endoplasmic reticulum of human liver and intestine, where its large, highly lipophilic active site binds a broad spectrum of substrates—including more than half of all marketed drugs—catalyzing the insertion of an oxygen atom into C–H or C=C bonds to increase polarity and facilitate elimination; expression is up-regulated by nuclear receptor PXR and down-regulated by inflammatory cytokines, leading to up to 40-fold inter-individual variability, and common genetic variants (e.g., *22) modestly alter activity, while potent inhibitors like ketoconazole or grapefruit furanocoumarins and inducers like rifampicin or St. John’s wort can precipitate clinical drug–drug interactions, making CYP3A4 a central determinant of systemic exposure, efficacy, and toxicity for statins, calcium-channel blockers, immunosuppressants, and many oncology agents.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CYP3A4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human liver. Anti-CYP3A4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
