WB result of Collagen I Recombinant Rabbit mAb
Primary antibody: Collagen I Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HFF whole cell lysate 20 µg
Negative control: HT-29 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 139 kDa
Observed MW: 220 kDa
Collagen I Recombinant Rabbit mAb (S-1473-5)
Collagen I Recombinant Rabbit mAb (S-1473-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Collagen I |
| Synonyms | Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Extracellular space, Extracellular matrix |
| Accession | P02452 |
| Clone Number | S-1473-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HFF |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
Collagen I protein is the most abundant collagen in the human body, making up around 90% of the total collagen in vertebrates. It is a major structural component of connective tissues, including skin, tendons, ligaments, bones, and the organic part of teeth. Collagen I is composed of a triple helix structure formed by two α1 chains and one α2 chain encoded by the COL1A1 and COL1A2 genes, respectively. This protein provides significant tensile strength and elasticity to tissues, allowing them to withstand mechanical stress. Its synthesis involves complex processes, including transcription, translation, post-translational modifications, and assembly into collagen fibers. Due to its high biocompatibility and unique physical properties, Collagen I is widely used in pharmaceuticals, regenerative medicine, and clinical applications.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Collagen I antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HFF cells (top panel) and negative staining in HT-29 cells (below panel). Anti-Collagen I antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
