WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: U-937 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Lane 4: HuT-78 whole cell lysate 20 µg
Lane 5: HUVEC whole cell lysate 20 µg
Lane 6: THP-1 whole cell lysate 20 µg
Lane 7: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
CD99 Mouse mAb (S-2121-45)
CD99 Mouse mAb (S-2121-45)
Price:
Regular price
$100.00 USD
Regular price
Sale price
$100.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD99 |
| Synonyms | CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; MIC2; MIC2X; MIC2Y |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | P14209 |
| Clone Number | S-2121-45 |
| Antibody Type | Mouse mAb |
| Isotype | IgM |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | U-937, Jurkat, Molt-4, HuT-78, HUVEC, THP-1, SH-SY5Y, mouse spleen, rat spleen, rat thymus |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
CD99, also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein encoded by the CD99 gene. It is widely expressed in various cell types, including hematopoietic cells, endothelial cells, and many types of cancer cells. This protein plays crucial roles in multiple biological processes such as cell adhesion, migration, differentiation, and apoptosis. In the immune system, CD99 is involved in T-cell activation, maturation, and the regulation of leukocyte transmigration. It also has a significant impact on tumor biology, as it is often overexpressed in cancers like Ewing sarcoma and certain leukemias. Engagement of CD99 by specific antibodies can induce caspase-independent cell death in tumor cells, making it a potential therapeutic target.
Picture
Picture
Western Blot
WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
WB result of CD99 Mouse mAb
Primary antibody: CD99 Mouse mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 27 kDa
Immunocytochemistry
ICC shows positive staining in Hut78 cells. Anti- CD99 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
