Flow cytometric analysis of Human peripheral blood cells labelling CD88 antibody at 1/500 (0.1 μg) dilution/ (Right panel) compared with a Rabbit IgG Isotype Control / (Left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 421™ antibody separately.
CD88 Recombinant Rabbit mAb (S-1921-43)
CD88 Recombinant Rabbit mAb (S-1921-43)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD88 |
| Synonyms | C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor (C5a-R; C5aR); C5AR; C5R1; C5AR1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P21730 |
| Clone Number | S-1921-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Predicted Reactivity | Cz |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
| FCM | 1:500 | Hu |
Background
CD88, also known as C5a receptor 1 (C5aR1), is a G protein-coupled receptor that plays a crucial role in the immune system. It is primarily expressed on various immune cells, including neutrophils, monocytes, macrophages, and some endothelial cells. The binding of the anaphylatoxin C5a to CD88 triggers a wide range of biological responses, such as chemotaxis, degranulation, and the release of pro-inflammatory cytokines, which are essential for the recruitment of immune cells to sites of infection or inflammation. Additionally, CD88-mediated signaling can enhance phagocytosis and promote the activation of the immune response. Dysregulation of CD88 function has been implicated in several inflammatory and autoimmune diseases, making it a potential therapeutic target for interventions aimed at modulating immune responses.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD88 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD88 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
