WB result of CD48 Recombinant Rabbit mAb
Primary antibody: CD48 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 38~45 kDa
CD48 Recombinant Rabbit mAb (S-1515-9)
CD48 Recombinant Rabbit mAb (S-1515-9)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD48 |
| Synonyms | CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2 (SLAMF2); Signaling lymphocytic activation molecule 2; TCT.1; BCM1; BLAST1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane, Secreted |
| Accession | P09326 |
| Clone Number | S-1515-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Positive Sample | Raji, Ramos |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:500 | Hu |
Background
CD48 is a protein that belongs to the signal lymphocyte activation molecule (SLAMF) family. It is a cell-surface glycoprotein that plays a significant role in immune cell adhesion and activation. CD48 is expressed on various hematopoietic cells, including T cells, B cells, natural killer (NK) cells, monocytes, and eosinophils. CD48 does not have an intracellular domain; instead, it is anchored to the cell surface via a glycosyl-phosphatidyl-inositol (GPI) linkage. This protein interacts with several ligands, including CD2, CD244 (2B4), and bacterial FimH, to mediate various immune functions. The interaction of CD48 with CD2 on T cells can promote T cell activation by facilitating the recruitment of signaling components to the T cell receptor (TCR). CD48 also contributes to the organization of the immune synapse, adhesion, and costimulation through its interaction with CD2. The interaction between CD48 and CD244 can regulate the activation of NK cells and CD8+ T effector cells. CD48 also plays a role in the immune response against bacteria by binding to the bacterial component FimH through mannose residues in the GPI linkage. In terms of cancer treatment, CD48 has been identified as a potential target in immunotherapy. It is highly expressed in certain types of cancer, such as renal clear cell carcinoma (ccRCC), and may be associated with tumor immune evasion.
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Picture
Western Blot
IP
CD48 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD48 in 0.4 mg Ramos whole cell lysate.
Western blot was performed on the immunoprecipitate using CD48 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Ramos whole cell lysate 20 µg (Input)
Lane 2: CD48 Rabbit mAb IP in Ramos whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Ramos whole cell lysate
Predicted MW: 28 kDa
Observed MW: 38~45 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD48 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
