WB result of CD352/SLAM6 Recombinant Rabbit mAb
Primary antibody: CD352/SLAM6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Daudi whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Negative control: U937 whole cell lysate; THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 45-65 kDa
CD352/SLAM6 Recombinant Rabbit mAb (SDT-2472-77)
CD352/SLAM6 Recombinant Rabbit mAb (SDT-2472-77)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD352/SLAM6 |
| Synonyms | SLAM family member 6; Activating NK receptor; NK-T-B-antigen (NTB-A); KALI |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q96DU3 |
| Clone Number | SDT-2472-77 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu |
| Positive Sample | Ramos, Jurkat, Daudi, Raji |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
| IHC-P | 1:200 | Hu |
| IF | 1:200 | Hu |
Background
CD352 (also known as SLAMF6, NTB-A, or LY108) is a type I transmembrane glycoprotein belonging to the signaling lymphocytic activation molecule (SLAM) family and a member of the CD2 subfamily of immunoregulatory receptors. This protein is expressed on the surface of natural killer (NK) cells, T cells (upregulated upon activation), and B cells, functioning through homophilic interactions to regulate NK cell cytotoxicity, T cell co-stimulation (independent of CD28), Th1 cytokine production, and B cell tolerance. The extracellular domain of CD352 contains Ig-like domains, while its intracellular region harbors conserved tyrosine-based signaling motifs that can be phosphorylated and recruit adaptor proteins such as SH2D1A (SAP) and SH2D1B (EAT-2), thereby modulating innate and adaptive immune responses. Studies indicate that reduced CD352 expression in multiple myeloma (MM) may be linked to its role in immune regulation and the tumor microenvironment. Additionally, CD352 activation promotes T cell proliferation and cytokine secretion while triggering cytolytic activity and signal transduction cascades in NK cells.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Non-Hodgkin’s lymphoma. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous carcinoma. Anti-CD352/SLAM6 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti- CD352/SLAM6 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti- CD352/SLAM6 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
