• Product image media
  • Product image media
  • Product image media
  • Product image media
  • Product image media
1 5

Caldesmon Recombinant Rabbit mAb (SDT-056-48)

Caldesmon Recombinant Rabbit mAb (SDT-056-48)

Catalog Number: S0B2048 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter
Share:
Price:
$131.00 SGD
$131.00 SGD
Size:
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Rabbit
Antigen Caldesmon
Synonyms CDM, CALD1, CAD
Immunogen Synthetic Peptide
Location Cytoskeleton, Intracellular
Accession Q05682
Clone Number SDT-056-48
Antibody Type Rabbit mAb
Application WB, IHC-P, ICC, ICFCM, IP
Reactivity Hu, Ms, Rt
Purification Protein A
Concentration 0.5mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage 12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
ICFCM 1:500
WB 1:1000
IHC-P 1:2000
IP 1:25
ICC 1:500

Background

Caldesmon is a binding protein of smooth actin and calmodulin, a cytoskeleton-associated protein present in thin filaments (smooth and striated muscles) that regulates the interaction of actin and myosin. It is available in low molecular weight (l-Caldesmon) and high molecular weight (h-Caldesmon). The latter is thought to be present only in visceral and vascular smooth muscle and myoepithelium. Caldesmon is commonly used in the diagnosis of smooth muscle differentiation tumors.

Picture

Western Blot

WB result of Caldesmon Rabbit mAb                

Primary antibody: Caldesmon Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate        

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: Lane 1 and lane 2 : 5s 
                         Lane 3 and lane 4 : 10s

WB result of Caldesmon Rabbit mAb                

Primary antibody: Caldesmon Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: 5s

FC

Flow cytometric analysis of MCF7 (left) / HeLa (right) cells labelling Caldesmon antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MCF7

IP

Caldesmon Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Caldesmon in 0.4mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Caldesmon Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : HeLa whole cell lysate 10µg (input)
Lane 2 : Caldesmon Rabbit mAb IP in HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: 60s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.              

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.              

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.              

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas cancer.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.              

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                    

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.                          

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control.IHC shows negativee staining in paraffin-embedded human skelteal muscle.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.              

Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells (top panel) and negative staining in MCF7 cells (below panel). Anti-Caldesmon antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).