Caldesmon Recombinant Rabbit mAb (SDT-056-48)

Catalog Number: S0B2048 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

Price:

$100.00 USD

Size: 25μl 100μl 500μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	Caldesmon
Synonyms	CDM, CALD1, CAD
Immunogen	Synthetic Peptide
Location	Cytoskeleton, Intracellular
Accession	Q05682
Clone Number	SDT-056-48
Antibody Type	Rabbit mAb
Application	WB, IHC-P, ICC, ICFCM, IP
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
ICFCM	1:500
WB	1:1000
IHC-P	1:2000
IP	1:25
ICC	1:500

Background

Caldesmon is a binding protein of smooth actin and calmodulin, a cytoskeleton-associated protein present in thin filaments (smooth and striated muscles) that regulates the interaction of actin and myosin. It is available in low molecular weight (l-Caldesmon) and high molecular weight (h-Caldesmon). The latter is thought to be present only in visceral and vascular smooth muscle and myoepithelium. Caldesmon is commonly used in the diagnosis of smooth muscle differentiation tumors.

Picture

Western Blot

WB result of Caldesmon Rabbit mAb

Primary antibody: Caldesmon Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: Lane 1 and lane 2 : 5s
Lane 3 and lane 4 : 10s

WB result of Caldesmon Rabbit mAb

Primary antibody: Caldesmon Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: 5s

FC

Flow cytometric analysis of MCF7 (left) / HeLa (right) cells labelling Caldesmon antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MCF7

IP

Caldesmon Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating Caldesmon in 0.4mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using Caldesmon Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : HeLa whole cell lysate 10µg (input)
Lane 2 : Caldesmon Rabbit mAb IP in HeLa whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 80 kDa
Observed MW: 80 kDa
Exposure time: 60s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon.

Anti-Caldesmon antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.