Flow cytometric analysis of Mouse CD95 expression on C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were stained with either Biotin Armenian hamster IgG Isotype Control (Black line histogram) or SDT Biotin Armenian hamster Anti-Mouse CD95 Antibody (Red line histogram) at 5 μl/test followed by Sav-iFluor PE, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Armenian hamster Anti-Mouse CD95 Antibody (S-R535)
Biotin Armenian hamster Anti-Mouse CD95 Antibody (S-R535)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD95 |
| Synonyms | Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; Apt1; Tnfrsf6; Fas |
| Location | Cell membrane |
| Accession | P25446 |
| Clone Number | S-R535 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse thymocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD95, also known as Fas or APO-1, is a 48 kDa type-I transmembrane receptor of the TNF-receptor superfamily that, upon trimerization by its cognate ligand CD95L (FasL), assembles the death-inducing signaling complex (DISC) by recruiting the adaptor FADD and procaspase-8/10, triggering a caspase cascade that culminates in apoptosis, thereby governing immune homeostasis, peripheral tolerance, and elimination of infected or oncogenic cells, while dysregulation of this pathway contributes to autoimmune lymphoproliferative syndrome, cancer immune evasion, and resistance to chemotherapy.
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