WB result of ARL13B Recombinant Rabbit mAb
Primary antibody: ARL13B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 55 kDa
ARL13B Recombinant Rabbit mAb (S-2113-11)
ARL13B Recombinant Rabbit mAb (S-2113-11)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ARL13B |
| Synonyms | ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1 (ARL2-like protein 1); ARL2L1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Cell membrane |
| Accession | Q3SXY8 |
| Clone Number | S-2113-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, 293T, NCCIT |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200-1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
| IF | 1:500 | Rt |
Background
ARL13B, also known as ADP-ribosylation factor-like protein 13B, is a small GTPase belonging to the ADP-ribosylation factor family and the Ras superfamily of GTPases. It is encoded by the ARL13B gene in humans and is predominantly localized in primary cilia. This protein plays a crucial role in cilia formation and maintenance. Mutations in the ARL13B gene can lead to Joubert syndrome, a ciliopathy characterized by brain malformations, polydactyly, and renal cyst formation. ARL13B is also involved in regulating intraflagellar transport and the localization of other proteins within the cilium, such as Smoothened (Smo), which is essential for Sonic hedgehog (Shh) signaling. Additionally, ARL13B has been found to promote the proliferation, migration, and osteogenesis of osteoblasts, suggesting its potential role in bone formation and mechanosensation.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-ARL13B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-ARL13B antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ARL13B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-ARL13B antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-ARL13B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti-ARL13B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded rat brain. Anti-ARL13B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
