Flow cytometric analysis of Human Glypican-3 expression on HepG2 cells. Cells from the HepG2 (Human hepatocellular carcinoma epithelial cell, Right) or A549 (Human lung carcinoma epithelial cell, Left) was stained with either APC Rabbit IgG Isotype Control (Black line histogram) or SDT APC Rabbit Anti-Human Glypican-3 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC Rabbit Anti-Human Glypican-3 Antibody (SDT-R032)
APC Rabbit Anti-Human Glypican-3 Antibody (SDT-R032)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glypican-3 |
| Synonyms | GTR2-2; Intestinal protein OCI-5; MXR7; OCI5; GPC3 |
| Location | Cell membrane |
| Accession | P51654 |
| Clone Number | SDT-R032 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | HepG2 |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
Glypican-3 (GPC3) is a cell-surface glycoprotein that is highly expressed in hepatocellular carcinoma (HCC) and some other cancers. It is a member of the glypican family of heparan sulfate proteoglycans, consisting of a core protein and heparan sulfate chains, and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. GPC3 is encoded by the GPC3 gene located on the X chromosome and is cleaved by furin into an N-terminal 40 kDa subunit and a C-terminal 30 kDa subunit. It plays a role in cell proliferation, morphogenesis, and oncogenesis by interacting with signaling pathways such as Wnt, Hedgehog, and fibroblast growth factor. Abnormal expression of GPC3 is associated with poor prognosis in cancer, making it a potential target for cancer diagnosis and immunotherapy.
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