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Anti-Rabbit and Mouse HRP&DAB IHC detection kit

Anti-Rabbit and Mouse HRP&DAB IHC detection kit

Catalog Number: S0C1001 Application: IHC-P Reactivity: Mouse,Rabbit Conjugation: HRP Brand: Starter
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$170 USD
$170 USD
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Product Details

Product Specification


Host Goat
Clone Number N/A
Antibody Type pAb
Application IHC-P
Reactivity Ms, Rb
Purification Protein A
Conjugation HRP
Physical Appearance Liquid
Stability & Storage

12 months from date of receipt, 2 to 8 °C as supplied

Dilution


application dilution species
IHC-P N/A

Background

Anti-Rabbit and Mouse HRP-DAB IHC detection kit utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround.
Prior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add OneStep polymer HRP Goat anti-Rabbit and Mouse IgG (H+L) (specific for IHC) and incubate for 15-30 minutes. Polymer HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.

Picture

Contents & Protocol

Immunohistochemistry protocol:

  1. Cut and mount sections on slides coated with a suitable tissue adhesive.
  2. De-paraffinize sections in xylene or xylene substitutes.
  3. Re-hydrate through graded alcohols.
  4. Wash slides in distilled H2O.
  5. Perform antigen retrieval as required.
  6. Wash slides in distilled H2O.
  7. Quench endogenous peroxidase using Hydrogen Peroxide Block for 5-10 minutes.
  8. Wash in wash buffer for 3 x 3 minutes.
  9. Incubate with optimally diluted primary antibody.
  10. Wash in wash buffer for 3 x 3 minutes.
  11. Incubate with ready to use Goat anti-Rabbit&Mouse HRP Conjugate for 15-30 minutes.
  12. Wash in wash buffer for 3 x 3 minutes.
  13. Add DAB working solution (DAB substrate diluted with DAB dilution buffer) until color development finish.
  14. Rinse slides in water.
  15. Add 100ul Haematoxylin and incubate for 3 minutes, then rinse slides in water.
  16. Clear and mount sections

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human stomach. Anti-Claudin18.2 antibody(S0B2069) was used at 1/1000 dilution, followed by anti-Rabbit and Mouse HRP-DAB IHC detection kit. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Claudin18.2 antibody(S0B2069) was used at 1/1000 dilution, followed by anti-Rabbit and Mouse HRP-DAB IHC detection kit. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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