alpha smooth muscle Actin (SMA) Recombinant Rabbit mAb (SDT-132-63)
alpha smooth muscle Actin (SMA) Recombinant Rabbit mAb (SDT-132-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | alpha smooth muscle Actin (SMA) |
| Synonyms | Alpha-actin-2, ACTA2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P62736 |
| Clone Number | SDT-132-63 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.0625 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | |
| IF | 1:125 | |
| WB | 1:2000-1:10000 |
Background
α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
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Western Blot
WB result of alpha smooth muscle Actin (SMA) Rabbit mAb
Primary antibody: alpha smooth muscle Actin (SMA) Rabbit mAb at 1/6250 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse colon lysate 20 µg
Negative control: mouse skeletal muscle
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Exposure time: 60 s
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric carcinoma. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse colon. Anti-alpha smooth muscle Actin (SMA) antibody was used at 1/125 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
