alpha-actinin-2 Recombinant Rabbit mAb (SDT-032-79)

Catalog Number: S0B0026 Application: WB, IHC-P, IP Reactivity: Hu, Ms, Rt Conjugation: Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	alpha-actinin-2
Synonyms	ACTN2
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	P35609
Clone Number	SDT-032-79
Antibody Type	Rabbit mAb
Application	WB, IHC-P, IP
Reactivity	Hu, Ms, Rt
Predicted Reactivity	Bv
Purification	Protein A
Concentration	0.25 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
IP	1:25	Ms

Background

Alpha-actinin-2 is a protein which in humans is encoded by the ACTN2 gene. This gene encodes an alpha-actinin isoform that is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs. The primary function of alpha-actinin-2 is to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis. The diverse functions of alpha-actinin-2 are reflected in the diverse clinical presentation of patients carrying ACTN2 mutations.

Picture

Western Blot

WB result of alpha-actinin-2 Rabbit mAb
Primary antibody: alpha-actinin-2 Rabbit mAb at 1/5000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 103 kDa
Observed MW: 103 kDa

WB result of alpha-actinin-2 Rabbit mAb
Primary antibody: alpha-actinin-2 Rabbit mAb at 1/5000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Lane 3: rat heart lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 103 kDa
Observed MW: 103 kDa

IP

alpha-actinin-2 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating alpha-actinin-2 in 0.4 mg mouse skeletal muscle lysate.
Western blot was performed on the immunoprecipitate using alpha-actinin-2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: mouse skeletal muscle lysate 10 µg (Input)
Lane 2: alpha-actinin-2 Rabbit mAb IP in mouse skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG IP in mouse skeletal muscle lysate
Predicted MW: 103 kDa
Observed MW: 103 kDa
Exposure time: 40 s

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human prostate. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cervix cancer. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse kidney. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat colon. Anti-alpha-actinin-2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.