WB result of ADAM10 Rabbit pAb
Primary antibody: ADAM10 Rabbit pAb at 1/1000 dilution
Lane 1: A-431 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 68, 90 kDa
(This blot was developed with high sensitivity substrate)
ADAM10 Rabbit polyclonal antibody
ADAM10 Rabbit polyclonal antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Disintegrin and metalloproteinase domain-containing protein 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | O14672 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Rt, Pg, Bv |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| ICC | 1:500 | |
| ICFCM | 1:50 |
Background
ADAM10 is a sheddase, and has a broad specificity for peptide hydrolysis reactions. ADAM10 cleaves ephrin, within the ephrin/eph complex, formed between two cell surfaces. When ephrin is freed from the opposing cell, the entire ephrin/eph complex is endocytosed. In neurons, ADAM10 is the most important enzyme, with α-secretase activity for proteolytic processing of the amyloid precursor protein. ADAM10, along with ADAM17, cleaves the ectodomain of the triggering receptor expressed on myeloid cells 2 (TREM2), to produce soluble TREM2 (sTREM2), which has been proposed as a CSF and sera biomarker of neurodegeneration. ADAM10 being a major determinant of HER2 shedding, the inhibition of which, may provide a novel therapeutic approach for treating breast cancer and a variety of other cancers with active HER2 signaling.
Picture
Picture
Western Blot
WB result of ADAM10 Rabbit pAb
Primary antibody: ADAM10 Rabbit pAb at 1/1000 dilution
Lane 1: RAW 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 68, 90 kDa
(This blot was developed with high sensitivity substrate)
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) labelling ADAM10 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
ADAM10 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ADAM10 in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using ADAM10 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A431 whole cell lysate 20 µg (Input)
Lane 2: ADAM10 Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 84 kDa
Observed MW: 68, 90 kDa
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti-ADAM10 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
