WB result of ABCA1 Recombinant Rabbit mAb
Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell unboiled RIPA lysate 20 µg
Lane 2: untreated HepG2 whole cell unboiled RIPA lysate 20 µg
Lane 3: HepG2 treated with 20 μM T0901317 for 16 hours whole cell unboiled RIPA lysate 20 µg
Lane 4: U-87 MG whole cell unboiled RIPA lysate 20 µg
Negative control: HCT 116 whole cell unboiled RIPA lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 254 kDa
Observed MW: 300 kDa
ABCA1 Recombinant Rabbit mAb (S-1887-25)
ABCA1 Recombinant Rabbit mAb (S-1887-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ABCA1 |
| Synonyms | Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1 (ABC-1; ATP-binding cassette 1); Cholesterol efflux regulatory protein; ABC1; CERP |
| Immunogen | Synthetic Peptide |
| Location | Endosome, Cell membrane |
| Accession | O95477 |
| Clone Number | S-1887-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, U-87 MG, mouse liver, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
ABCA1 (ATP Binding Cassette Subfamily A Member 1) is a protein encoded by the ABCA1 gene, located on chromosome 9q31.1. It is a member of the ATP-binding cassette (ABC) transporter family, which uses energy from ATP hydrolysis to transport various substrates across cell membranes. ABCA1 specifically facilitates the efflux of cholesterol and phospholipids from cells to extracellular acceptors such as apolipoprotein A-I (APOA1), playing a crucial role in the formation of high-density lipoprotein (HDL) particles. This process is essential for reverse cholesterol transport (RCT), which helps remove excess cholesterol from tissues and prevents atherosclerosis. Mutations in the ABCA1 gene can lead to Tangier disease, a rare genetic disorder characterized by extremely low levels of HDL cholesterol and cholesterol accumulation in tissues.
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Western Blot
WB result of ABCA1 Recombinant Rabbit mAb
Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 254 kDa
Observed MW: 300 kDa
This blot was developed with high sensitivity substrate
WB result of ABCA1 Recombinant Rabbit mAb
Primary antibody: ABCA1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 254 kDa
Observed MW: 290 kDa
This blot was developed with high sensitivity substrate
