WB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: Caco-2 whole cell lysate 20 µg
Lane 5: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDa
GPD2 Recombinant Rabbit mAb (S-2941-41)
GPD2 Recombinant Rabbit mAb (S-2941-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GPD2 |
| Synonyms | Glycerol-3-phosphate dehydrogenase, mitochondrial; GPD-M; GPDH-M; mitohondrial glycerophosphate dehydrogenase gene (mGDH); mtGPD |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P43304 |
| Clone Number | S-2941-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, MCF7, U-87 MG, Caco-2, LNCaP, NIH/3T3, mouse skeletal muscle, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu |
Background
GPD2 (glycerol-3-phosphate dehydrogenase 2, mitochondrial) is a 727-amino-acid, FAD-binding inner-mitochondrial-membrane enzyme that catalyzes the irreversible oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate, feeding electrons into the respiratory chain at coenzyme Q and thereby serving as the rate-limiting component of the glycerol-phosphate shuttle that re-oxidizes cytosolic NADH to sustain glycolysis; it is expressed ubiquitously, its activity modulates glucose-stimulated insulin secretion, reactive-oxygen-species generation, platelet activation and bleeding risk, and its gene (chr2q24.1) produces two transcripts encoding the same protein.
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Western Blot
WB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDa
WB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDa
WB result of GPD2 Recombinant Rabbit mAb
Primary antibody: GPD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 81 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast squamous cell carcinoma. Anti-GPD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
