Standard curve
Example of Human PCSK9 standard curve in Assay Diluent #1.
Human & Monkey PCSK9 OneStep ELISA Kit
Human & Monkey PCSK9 OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | PCSK9 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.3%； Inter-assay: 5.2% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 11.177 pg/ml |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Recovery | Cell culture supernatant: 85% Serum: 100% Plasma: 87% |
| Assay time | 60 minutes |
| Species reactivity | Human; Monkey |
Background
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a key regulator of cholesterol metabolism, primarily known for its role in degrading low-density lipoprotein receptors (LDLR) in the liver. By binding to LDLR and promoting its lysosomal degradation, PCSK9 reduces the liver's ability to clear circulating LDL cholesterol (LDL-C), leading to elevated plasma LDL-C levels. Gain-of-function mutations in the PCSK9 gene are associated with autosomal dominant hypercholesterolemia, while loss-of-function variants correlate with reduced LDL-C and cardiovascular risk.
Picture
Picture
ELISA
Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: HepG2 human hepatocellular carcinoma cells culture supernatant (5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 25.61 ng/mL in HepG2 cells culture supernatant.
Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human serum (1%) and human plasma (1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 465.62 ng/mL in human serum. The mean target concentration was determined to be 482.06 ng/mL in human serum .
Linearity
The concentrations of Human PCSK9 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: female monkey serum (2%) and male monkey serum (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 91.66 ng/mL in female monkey serum. The mean target concentration was determined to be 98.79 ng/mL in male monkey serum.
Specificity
Test the specificity of different serum.
To assess the linearity of the assay, sample was spiked with high concentrations of Human PCSK9 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human PCSK9 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram
