| Species | Streptococcus pyogenes |
| Synonyms | Endo-beta-N-acetylglucosaminidase EndoS, Endoglycosidase S, EndoS |
| Expression System | E.coli |
| Molecular Weight | 109 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl、50 mM NaCl、5 mM EDTA（pH 7.5 @ 25°C） |
| Stability & Storage |
Store at -25 ~ -15℃
for 2 years
|
| Reference |
[1] Collin,M.EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG[J].Embo Journal, 2014, 20(12):3046-3055. [2] Beatriz,Trastoy,Joseph,et al.Liquid–liquid
diffusion crystallization improves the X-ray diffraction of EndoS, an
endo-β-N-acetylglucosaminidase from Streptococcus pyogenes with activity on
human IgG[J].Acta Crystallographica, 2013. |
Anenzyme expressed by Streptococcus pyogenes capable of releasing the
terminal sialic acid residues from glycoproteins such as immunoglobulins.Specifically catalyzes the hydrolysis of the beta-1,4 linkage between
the first two N-acetylglucosamine residues of the complex-type N-linked glycan
located on 'Asn-297' of the Fc region of IgG antibodies (IGHG1, IGHG2, IGHG3 or
IGHG4), thereby preventing interaction between IgGs and Fc receptors and
ability to activate the complement pathway. Endo S is an endoglycosidase
specific for cleaving the N-linked glycans from the chitobiose core of the
heavy chain of native IgG.
Storage Solution : 200U/ul Endo S、20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA. (pH 7.5 @ 25°C)
10*Reaction Buffer：50mM CaCl2、500 mM sodium acetate (pH 5.5 @ 25°C)
1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.
2. Add 1 µl Endo S.
3. Incubate reaction at 37°C for 1 hour
The experimental design added 10 U, 2 U, 1 U, 0.5 U, 0.4U and 0.25 U enzymes into the 20 ul reaction system to investigate the effect of Endo S on the digestion of human IgG.
M marker
Lan1 human IgG 1μg
Lan2 human IgG 1μg+10 U Endo S
Lan3 human IgG 1μg+2 U Endo S
Lan4 human IgG 1μg+1 U Endo S
Lan5 human IgG 1μg+0.5 U Endo S
Lan6 human IgG 1μg+0.4 U Endo S
Lan7 human IgG 1μg+0.25 U Endo S
