CTLA-4 Fc Chimera Protein, Human

Catalog Number: UA010039 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$210 USD

Size: 100μg 500μg

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Product Details

Product Specification

Species	Human
Accession	P16410
Amino Acid Sequence	Ala37-Phe162, with C-terminal Human IgG1 Fc
Expression System	HEK293
Molecular Weight	49-54 kDa(Reducing)
Purity	＞95%, by SDS-PAGE under reducing conditions ＞95%，as determined by HPLC
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	Human Fc Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.

Background

Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. The family includes CD28, CTLA-4 and ICOS as well as other proteins including PD-1, BTLA and TIGIT.Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. The mechanisms by which CTLA-4 exerts its inhibitory function can be categorized as either cell-intrinsic (affects the CTLA-4 expressing T-cell) or cell-extrinsic (affects secondary cells). CTLA-4 mainly acts in a cell-extrinsic manner via its competition with CD28, CTLA-4-mediated trans-endocytosis of CD80 and CD86, and its direct tolerogenic effects on the interacting cell.

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Bioactivity

Anti-His antibody Immobilized on CM5 Chip captured B7-1/CD80 His Tag, Human (Cat. No. UA010085), can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with an affinity constant of 8.432nM as determined in SPR assay (Biacore T200).

Anti-His antibody Immobilized on CM5 Chip captured B7-2/CD86 His Tag, Human (Cat. No. UA010098), can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with an affinity constant of 49.58nM as determined in SPR assay (Biacore T200).

Immobilized B7-1/CD80 His Tag, Human (Cat. No. UA010085) at 5μg/mL (100 μL/well) can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with EC50 of 85.96ng/mL.

Immobilized B7-2/CD86 His Tag, Human (Cat. No. UA010098) at 5μg/mL (100μL/well) can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with EC50 of 33.50ng/mL.