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IL-3β Protein, Rat

IL-3β Protein, Rat

Catalog Number: UA040225 Reactivity: Rat Conjugation: Unconjugated Brand: UA BIOSCIENCE
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$235.00 SGD
$235.00 SGD
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Product Details

Product Specification


Species Rat
Synonyms Hematopoietic Growth Factor; MCGF; Multipotential Colony-stimulating Factor; P-cell-stimulating Factor
Accession P97688
Amino Acid Sequence Met26-Cys169
Expression System E.coli
Molecular Weight 16kDa (Reducing)
Purity ＞95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4.
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Mangi, M.H. and A.C. Newland (1999) Cytokines Cell. Mol. Ther. 5:87.
Esandi, M. del C. et al. (1998) Gene 211:151.
Cohen, D.R. et al. (1986) Nucleic Acids Res. 14:3641.
Gebicke-Haerter, P.J. et al. (1994) J. Neuroimmunol. 50:203.

Background

Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. It exerts its biological activities through binding to Interleukin-3 receptors included α and β subunits, but sequence analysis revealed a number of features indicative of the presence of only one β-subunit in the rat. Interleukin-3 acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Furthermore, rat and human IL-3 share low homology and have not cross species activity.

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Bioactivity

Measured in a cell proliferation assay using murine MC/9 cells cells. The EC50 this effect is less than 7ng/ml.

SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).

RP-HPLC

>95% as determined by RP-HPLC