Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 1ng/ml.
FGF-1/FGF-Acidic Protein, Bovine
FGF-1/FGF-Acidic Protein, Bovine
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Product Details
Product Details
Product Specification
| Species | Bovine |
| Synonyms | Fibroblast growth factor 1; FGF-1; Acidic eye-derived growth factor II (EDGF II); Acidic fibroblast growth factor (aFGF); Endothelial cell growth factor (ECGF); Heparin-binding growth factor 1 (HBGF-1); Prostatropin; |
| Accession | P03968 |
| Amino Acid Sequence | Ala2-Asp155 |
| Expression System | E.coli |
| Molecular Weight | 18 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Nat Rev Drug Discov. 2009 Mar;8(3):235-53. |
Background
Fibroblast Growth Factor-Acidic (FGF-Acidic), also known as FGF-1, is a member of the fibroblast growth factor family that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and angiogenesis. FGF-Acidic is a potent mitogen that stimulates the growth and survival of a wide range of cell types, including fibroblasts, endothelial cells, and smooth muscle cells. It is involved in tissue repair, wound healing, and embryonic development.
Bovine FGF-Acidic refers to the version of this growth factor derived from bovine (cow) sources. Bovine FGF-Acidic shares high sequence homology (92%) with human FGF-Acidic and exhibits similar biological activities.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
>95% as determined by SEC-HPLC.
