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FGF-1/FGF-Acidic Protein, Bovine

FGF-1/FGF-Acidic Protein, Bovine

Catalog Number: UA040311 Reactivity: Bovine Conjugation: Unconjugated Brand: UA BIOSCIENCE
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$79.00 SGD
$79.00 SGD
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Product Details

Product Specification


Species Bovine
Synonyms Fibroblast growth factor 1; FGF-1; Acidic eye-derived growth factor II (EDGF II); Acidic fibroblast growth factor (aFGF); Endothelial cell growth factor (ECGF); Heparin-binding growth factor 1 (HBGF-1); Prostatropin;
Accession P03968
Amino Acid Sequence

Ala2-Asp155
Expression System E.coli
Molecular Weight

18 kDa (Reducing)
Purity >95% by SDS-PAGE and HPLC.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag No Tag
Physical Appearance Lyophilized Powder
Storage Buffer 20mM Tris, 200mM NaCl, pH8.0
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

Nat Rev Drug Discov. 2009 Mar;8(3):235-53.

Background

Fibroblast Growth Factor-Acidic (FGF-Acidic), also known as FGF-1, is a member of the fibroblast growth factor family that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and angiogenesis. FGF-Acidic is a potent mitogen that stimulates the growth and survival of a wide range of cell types, including fibroblasts, endothelial cells, and smooth muscle cells. It is involved in tissue repair, wound healing, and embryonic development.
Bovine FGF-Acidic refers to the version of this growth factor derived from bovine (cow) sources. Bovine FGF-Acidic shares high sequence homology (92%) with human FGF-Acidic and exhibits similar biological activities.

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Bioactivity

Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 1ng/ml.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

>95% as determined by SEC-HPLC.